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KMID : 0861020060210020129
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Korea Journal of Herbology
2006 Volume.21 No. 2 p.129 ~ p.133
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Identification of Cervus elaphus Species by Sequencing Analysis and BLAST Search
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Seo Jung-Chul
Kim Min-Jung
Lee Chan
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Abstract
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Objectives: Cervus elaphus species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Cenvus elaphus species could be identified by sequencing analysis and to verify Basic Local Alignment Search Tool (BLAST) search, which was used to assess genetic identification.
Methods: The DNAs of Cervus elaphus species were extracted, amplified by PCR, and sequenced. The DNAs of Cervus species were identified by BLAST search in website.
Results: By BLAST search one of Cervus elaphus species was identified as Cervus elaphussibericus but the other was identified as Cervus elaphus nelsoni. This work showed that identification can efficiently be performed by BLAST search.
Conclusions: These results suggest that sequencing following BLAST search might be able to provide the identification of Cervus elaphus species.
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KEYWORD
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sequencing, genetic identification, Cervus elaphus species
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